UM-Dearborn

Protocols

• Agarose gel electrophoresis protocol

• Community DNA isolation protocol for marine sediments

• DNA extraction protocol for Gram negative bacteria

• DNA extraction protocol for Gram positive bacteria

• Protocol for dsrAB PCR amplification

• Protocol for nirK PCR amplification

• Resuscitation and cultivation of extreme halophiles

• Sequencing protocol

• Soil DNA isolation protocol

• RNA isolation from a variety of biological sources

• Linearizarion of miniprep plasmid DNA

• Purification of linearized plasmids

• In vitro transcription protocol

• Purification of RNA from in vitro transcription

• Analyzing nucleic acids using Bioanalyzer

• Quantifying RNA by spectral absorption

• RNA analysis on non-denaturing agarose gel electroporesis

• DNA quantification using sybr green and a microplate reader

• Carbon monoxide dehydrogenase assay

• Formate dehydrogenase assay

• Butanol dehydrogenase assay

• Hydrogenase assay
  
  

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