Pat Lenderman's Lab Page



 



Former Project: mEmbryonic Stem Cells at the Barald Lab


Protocols (Under Construction)


Tissue Culture


Passing ES Cells 

Freezing 

Thawing 

Plating Cells  

Harvesting and Counting  


Immunohistochemistry


Immunohistochemistry   This is my secret protocol for immunohistochemistry made up with the help of Kate Barald, Zymed, and trial and error. It produces brilliantly positive labeled cells and neurons against a completely negative background.

 


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Recipes



ES Medium 

Differentiation Medium 

CHO Medium 

UIMO Medium 


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Photomicrography


Nikon ACT Software 

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PCR (coming soon)


Reagents (Coming Soon)

Genomic DNA Isolation (Coming Soon)

Reading The Optical Density (Coming Soon)

PCR Reagent Prep (Coming Soon)

PCR (Coming Soon)

Gel  (Coming Soon)

Electrophoresis (Coming Soon)

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Western Blotting (coming soon)


Protein Isolation (Coming Soon)

Bradford Assay (Coming Soon)

Immunoprecipitation (Coming Soon)

Western Blot (Coming Soon)


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Misc.


Figuring Standard Deviation in Excel 


Coulter Counter Threshold Settings  


Figuring Regression Value 


Coulter Counter Set Up 



Room and Phone numbers  (Coming Soon)




 

 

Research is to see what everybody else has seen, and to think what nobody else has thought.
- Albert-Szent Györgi

 

Last Updated: 06/28/08

 

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